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Strategy for successful expression of the Pseudomonas putida nitrile hydratase activator P14K in Escherichia coli
BACKGROUND: Activators of Nitrile hydratase (NHase) are essential for functional NHase biosynthesis. However, the activator P14K in P. putida is difficult to heterogeneously express, which retards the clarification of the mechanism of P14K involved in the maturation of NHase. Although a strep tag co...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3680314/ https://ncbi.nlm.nih.gov/pubmed/23731949 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-13-48 |
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