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Strategy for successful expression of the Pseudomonas putida nitrile hydratase activator P14K in Escherichia coli
BACKGROUND: Activators of Nitrile hydratase (NHase) are essential for functional NHase biosynthesis. However, the activator P14K in P. putida is difficult to heterogeneously express, which retards the clarification of the mechanism of P14K involved in the maturation of NHase. Although a strep tag co...
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| 主要な著者: | , , , , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
BioMed Central
2013
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3680314/ https://ncbi.nlm.nih.gov/pubmed/23731949 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-13-48 |
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