Strategy for successful expression of the Pseudomonas putida nitrile hydratase activator P14K in Escherichia coli

BACKGROUND: Activators of Nitrile hydratase (NHase) are essential for functional NHase biosynthesis. However, the activator P14K in P. putida is difficult to heterogeneously express, which retards the clarification of the mechanism of P14K involved in the maturation of NHase. Although a strep tag co...

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Autors principals: Liu, Yi, Cui, Wenjing, Fang, Yueqin, Yu, Yuechun, Cui, Youtian, Xia, Yuanyuan, Kobayashi, Michihiko, Zhou, Zhemin
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2013
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3680314/
https://ncbi.nlm.nih.gov/pubmed/23731949
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-13-48
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