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Investigation of tryptophan–NADH interactions in live human cells using three-photon fluorescence lifetime imaging and Förster resonance energy transfer microscopy

A method to investigate the metabolic activity of intracellular tryptophan (TRP) and coenzyme-NADH using three-photon (3P) fluorescence lifetime imaging (FLIM) and Förster resonance energy transfer (FRET) is presented. Through systematic analysis of FLIM data from tumorigenic and nontumorigenic cell...

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Detalhes bibliográficos
Main Authors: Jyothikumar, Vinod, Sun, Yuansheng, Periasamy, Ammasi
Formato: Artigo
Idioma:Inglês
Publicado em: Society of Photo-Optical Instrumentation Engineers 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3675329/
https://ncbi.nlm.nih.gov/pubmed/23748699
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1117/1.JBO.18.6.060501
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