Physiological fluorescence lifetime imaging microscopy improves Förster resonance energy transfer detection in living cells

Accurate, unambiguous detection of molecular interactions in living cells via measurements of Förster (or fluorescence) resonance energy transfer (FRET) events is experimentally challenging. We develop and apply a physiological fluorescence lifetime imaging microscopy (physiological FLIM) system to...

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Bibliographic Details
Main Authors: Chang, Ching-Wei, Wu, Mei, Merajver, Sofia D., Mycek, Mary-Ann
Format: Artigo
Language:Inglês
Published: Society of Photo-Optical Instrumentation Engineers 2009
Subjects:
JBO Letters
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2787065/
https://ncbi.nlm.nih.gov/pubmed/20059233
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1117/1.3257254
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