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Physiological fluorescence lifetime imaging microscopy improves Förster resonance energy transfer detection in living cells

Accurate, unambiguous detection of molecular interactions in living cells via measurements of Förster (or fluorescence) resonance energy transfer (FRET) events is experimentally challenging. We develop and apply a physiological fluorescence lifetime imaging microscopy (physiological FLIM) system to...

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Autori principali: Chang, Ching-Wei, Wu, Mei, Merajver, Sofia D., Mycek, Mary-Ann
Natura: Artigo
Lingua:Inglês
Pubblicazione: Society of Photo-Optical Instrumentation Engineers 2009
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2787065/
https://ncbi.nlm.nih.gov/pubmed/20059233
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1117/1.3257254
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