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Measuring protein interactions using Förster resonance energy transfer and fluorescence lifetime imaging microscopy
The method of fluorescence lifetime imaging microscopy (FLIM) is a quantitative approach that can be used to detect Förster Resonance Energy Transfer (FRET). The use of FLIM to measure the FRET that results from the interactions between proteins labeled with fluorescent proteins (FPs) inside living...
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2013
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3838496/ https://ncbi.nlm.nih.gov/pubmed/23806643 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2013.06.017 |
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