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Measuring protein interactions using Förster resonance energy transfer and fluorescence lifetime imaging microscopy

The method of fluorescence lifetime imaging microscopy (FLIM) is a quantitative approach that can be used to detect Förster Resonance Energy Transfer (FRET). The use of FLIM to measure the FRET that results from the interactions between proteins labeled with fluorescent proteins (FPs) inside living...

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Detalhes bibliográficos
Autor principal: Day, Richard N.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3838496/
https://ncbi.nlm.nih.gov/pubmed/23806643
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2013.06.017
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