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Additional correction for energy transfer efficiency calculation in filter-based Förster resonance energy transfer microscopy for more accurate results
Förster resonance energy transfer (FRET) microscopy is commonly used to monitor protein interactions with filter-based imaging systems, which require spectral bleedthrough (or cross talk) correction to accurately measure energy transfer efficiency (E). The double-label (donor+acceptor) specimen is e...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Society of Photo-Optical Instrumentation Engineers
2010
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2874045/ https://ncbi.nlm.nih.gov/pubmed/20459222 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1117/1.3407655 |
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