Three-Color Confocal Förster (or fluorescence) Resonance Energy Transfer Microscopy: Quantitative Analysis of Protein Interactions in the Nucleation of Actin Filaments in Live Cells()

Experiments using live cell 3-color FRET microscopy and corresponding in vitro biochemical reconstitution of the same proteins were conducted to evaluate actin filament nucleation A novel application of 3-color FRET data is demonstrated, extending the analysis beyond the customary energy transfer ef...

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Veröffentlicht in:Cytometry A
Hauptverfasser: Wallrabe, Horst, Sun, Yuansheng, Fang, Xiaolan, Periasamy, Ammasi, Bloom, George S.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2015
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4452401/
https://ncbi.nlm.nih.gov/pubmed/25755111
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cyto.a.22651
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