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Additional correction for energy transfer efficiency calculation in filter-based Förster resonance energy transfer microscopy for more accurate results

Förster resonance energy transfer (FRET) microscopy is commonly used to monitor protein interactions with filter-based imaging systems, which require spectral bleedthrough (or cross talk) correction to accurately measure energy transfer efficiency (E). The double-label (donor+acceptor) specimen is e...

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מידע ביבליוגרפי
Main Authors: Sun, Yuansheng, Periasamy, Ammasi
פורמט: Artigo
שפה:Inglês
יצא לאור: Society of Photo-Optical Instrumentation Engineers 2010
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC2874045/
https://ncbi.nlm.nih.gov/pubmed/20459222
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1117/1.3407655
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