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Trp replacements for tightly interacting Gly–Gly pairs in LacY stabilize an outward-facing conformation
Trp replacements for conserved Gly–Gly pairs between the N- and C-terminal six-helix bundles on the periplasmic side of lactose permease (LacY) cause complete loss of transport activity with little or no effect on sugar binding. Moreover, the detergent-solubilized mutants exhibit much greater therma...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
National Academy of Sciences
2013
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3670396/ https://ncbi.nlm.nih.gov/pubmed/23671103 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1306849110 |
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