Single Molecule Imaging of Transcription Factor Binding to DNA in Live Mammalian Cells

Imaging single fluorescent proteins in living mammalian cells is challenging due to out-of-focus fluorescence excitation by common microscopy schemes. We report the development of a novel fluorescence microscopy method, reflected light sheet microscopy (RLSM), which allows selective plane illuminati...

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Bibliografiske detaljer
Main Authors: Gebhardt, J Christof M, Suter, David M, Roy, Rahul, Zhao, Ziqing W, Chapman, Alec R, Basu, Srinjan, Maniatis, Tom, Xie, X Sunney
Format: Artigo
Sprog:Inglês
Udgivet: 2013
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3664538/
https://ncbi.nlm.nih.gov/pubmed/23524394
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2411
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