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Single Molecule Imaging of Transcription Factor Binding to DNA in Live Mammalian Cells
Imaging single fluorescent proteins in living mammalian cells is challenging due to out-of-focus fluorescence excitation by common microscopy schemes. We report the development of a novel fluorescence microscopy method, reflected light sheet microscopy (RLSM), which allows selective plane illuminati...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3664538/ https://ncbi.nlm.nih.gov/pubmed/23524394 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2411 |
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