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Probing Transcription Factor Dynamics at the Single-Molecule Level in a Living Cell
Transcription factors regulate gene expression through their binding to DNA. In a living Escherichia coli cell, we directly observed specific binding of a lac repressor, labeled with a fluorescent protein, to a chromosomal lac operator. Using single-molecule detection techniques, we measured the kin...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2853898/ https://ncbi.nlm.nih.gov/pubmed/17525339 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/science.1141967 |
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