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Misplaced helix slows down ultrafast pressure-jump protein folding
Using a newly developed microsecond pressure-jump apparatus, we monitor the refolding kinetics of the helix-stabilized five-helix bundle protein λ*YA, the Y22W/Q33Y/G46,48A mutant of λ-repressor fragment 6–85, from 3 μs to 5 ms after a 1,200-bar P-drop. In addition to a microsecond phase, we observe...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
National Academy of Sciences
2013
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3657825/ https://ncbi.nlm.nih.gov/pubmed/23620522 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1219163110 |
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