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Misplaced helix slows down ultrafast pressure-jump protein folding

Using a newly developed microsecond pressure-jump apparatus, we monitor the refolding kinetics of the helix-stabilized five-helix bundle protein λ*YA, the Y22W/Q33Y/G46,48A mutant of λ-repressor fragment 6–85, from 3 μs to 5 ms after a 1,200-bar P-drop. In addition to a microsecond phase, we observe...

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Main Authors: Prigozhin, Maxim B., Liu, Yanxin, Wirth, Anna Jean, Kapoor, Shobhna, Winter, Roland, Schulten, Klaus, Gruebele, Martin
格式: Artigo
語言:Inglês
出版: National Academy of Sciences 2013
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3657825/
https://ncbi.nlm.nih.gov/pubmed/23620522
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1219163110
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