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Short donor site sequences inserted within the intron of beta-globin pre-mRNA serve for splicing in vitro.

We constructed SP6-human beta-globin derivative plasmids that included possible donor site (5' splice site) sequences at a specified position within the first intron. The runoff transcripts from these templates truncated in the second exon were examined for splicing in a nuclear extract from He...

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Bibliographische Detailangaben
Hauptverfasser: Mayeda, A, Ohshima, Y
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1988
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC365523/
https://ncbi.nlm.nih.gov/pubmed/3185558
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