Short donor site sequences inserted within the intron of beta-globin pre-mRNA serve for splicing in vitro.
We constructed SP6-human beta-globin derivative plasmids that included possible donor site (5' splice site) sequences at a specified position within the first intron. The runoff transcripts from these templates truncated in the second exon were examined for splicing in a nuclear extract from HeLa ce...
Enregistré dans:
| Publié dans: | Mol Cell Biol |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Taylor & Francis
1988
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC365523/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3185558/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.8.10.4484-4491.1988 |
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