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Short donor site sequences inserted within the intron of beta-globin pre-mRNA serve for splicing in vitro.

We constructed SP6-human beta-globin derivative plasmids that included possible donor site (5' splice site) sequences at a specified position within the first intron. The runoff transcripts from these templates truncated in the second exon were examined for splicing in a nuclear extract from HeLa ce...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:Mol Cell Biol
Hauptverfasser: Mayeda, A, Ohshima, Y
Format: Artigo
Sprache:Inglês
Veröffentlicht: Taylor & Francis 1988
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC365523/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3185558/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.8.10.4484-4491.1988
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