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Determination of the number of proteins bound non-specifically to DNA
We have determined the change in the number of proteins bound non-specifically to DNA as a function of applied force using force–extension measurements on tethered DNA. Using magnetic tweezers, single molecules of λ DNA were repeatedly stretched and relaxed in the absence and presence of 170 nM λ re...
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| Autori principali: | , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2010
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3653182/ https://ncbi.nlm.nih.gov/pubmed/21386587 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1088/0953-8984/22/41/414104 |
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