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Determination of the number of proteins bound non-specifically to DNA

We have determined the change in the number of proteins bound non-specifically to DNA as a function of applied force using force–extension measurements on tethered DNA. Using magnetic tweezers, single molecules of λ DNA were repeatedly stretched and relaxed in the absence and presence of 170 nM λ re...

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Detalhes bibliográficos
Main Authors: Liebesny, Paul, Goyal, Sachin, Dunlap, David, Family, Fereydoon, Finzi, Laura
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3653182/
https://ncbi.nlm.nih.gov/pubmed/21386587
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1088/0953-8984/22/41/414104
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