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Target Identification by Diazirine Photo-Cross-linking and Click Chemistry

Target identification of biologically active small-molecules is often the rate-determining step in forward chemical genetics. Photo-affinity labeling (PAL) represents a useful biochemical strategy for target identification in complex protein mixtures. This unit describes the use of alkyl diazirine-b...

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Dettagli Bibliografici
Autori principali: MacKinnon, Andrew L., Taunton, Jack
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2009
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3647699/
https://ncbi.nlm.nih.gov/pubmed/23667793
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/9780470559277.ch090167
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