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Harnessing mutagenic homologous recombination for targeted mutagenesis in vivo by TaGTEAM
A major hurdle to evolutionary engineering approaches for multigenic phenotypes is the ability to simultaneously modify multiple genes rapidly and selectively. Here, we describe a method for in vivo-targeted mutagenesis in yeast, targeting glycosylases to embedded arrays for mutagenesis (TaGTEAM). B...
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| Autori principali: | , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
2013
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3643572/ https://ncbi.nlm.nih.gov/pubmed/23470991 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt150 |
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