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Shuffled antibody libraries created by in vivo homologous recombination and yeast surface display
Homologous recombination in yeast can be exploited to reliably generate libraries of >10(7) transformants from a pool of PCR products and a linearized plasmid vector. Homology in the PCR insertion products drives shuffling of these genes in vivo by yeast homologous recombination. Two scFvs that s...
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| Auteurs principaux: | , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Oxford University Press
2004
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC373425/ https://ncbi.nlm.nih.gov/pubmed/14978223 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh030 |
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