Загрузка...
Shuffled antibody libraries created by in vivo homologous recombination and yeast surface display
Homologous recombination in yeast can be exploited to reliably generate libraries of >10(7) transformants from a pool of PCR products and a linearized plasmid vector. Homology in the PCR insertion products drives shuffling of these genes in vivo by yeast homologous recombination. Two scFvs that s...
Сохранить в:
| Главные авторы: | , , |
|---|---|
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Oxford University Press
2004
|
| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC373425/ https://ncbi.nlm.nih.gov/pubmed/14978223 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh030 |
| Метки: |
Добавить метку
Нет меток, Требуется 1-ая метка записи!
|