Shuffled antibody libraries created by in vivo homologous recombination and yeast surface display
Homologous recombination in yeast can be exploited to reliably generate libraries of >10(7) transformants from a pool of PCR products and a linearized plasmid vector. Homology in the PCR insertion products drives shuffling of these genes in vivo by yeast homologous recombination. Two scFvs that shar...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC373425/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14978223/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnh030 |
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