Shuffled antibody libraries created by in vivo homologous recombination and yeast surface display

Homologous recombination in yeast can be exploited to reliably generate libraries of >10(7) transformants from a pool of PCR products and a linearized plasmid vector. Homology in the PCR insertion products drives shuffling of these genes in vivo by yeast homologous recombination. Two scFvs that shar...

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Detalles Bibliográficos

Publicado en:	Nucleic Acids Res
Principais autores:	Swers, Jeffrey S., Kellogg, Brenda A., Wittrup, K. Dane
Formato:	Artigo
Idioma:	Inglês
Publicado:	Oxford University Press 2004
Assuntos:	NAR Methods Online
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC373425/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14978223/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnh030
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