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Single-molecule colocalization FRET evidence that spliceosome activation precedes stable approach of 5′ splice site and branch site

Removal of introns from the precursors to messenger RNA (pre-mRNAs) requires close apposition of intron ends by the spliceosome, but when and how apposition occurs is unclear. We investigated the process by which intron ends are brought together using single-molecule fluorescence resonance energy tr...

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Detalhes bibliográficos
Main Authors: Crawford, Daniel J., Hoskins, Aaron A., Friedman, Larry J., Gelles, Jeff, Moore, Melissa J.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3637735/
https://ncbi.nlm.nih.gov/pubmed/23569281
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1219305110
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