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Single-molecule colocalization FRET evidence that spliceosome activation precedes stable approach of 5′ splice site and branch site
Removal of introns from the precursors to messenger RNA (pre-mRNAs) requires close apposition of intron ends by the spliceosome, but when and how apposition occurs is unclear. We investigated the process by which intron ends are brought together using single-molecule fluorescence resonance energy tr...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
National Academy of Sciences
2013
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3637735/ https://ncbi.nlm.nih.gov/pubmed/23569281 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1219305110 |
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