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Visualizing the splicing of single pre-mRNA molecules in whole cell extract
The excision of introns from nascent eukaryotic transcripts is catalyzed by the spliceosome, a highly complex and dynamic macromolecular machine composed of RNA and protein. Because of its complexity, biochemical analysis of the spliceosome has been previously limited to bulk assays in largely unfra...
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| Główni autorzy: | , , , , |
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| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
Cold Spring Harbor Laboratory Press
2008
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| Hasła przedmiotowe: | |
| Dostęp online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2151038/ https://ncbi.nlm.nih.gov/pubmed/18025254 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.794808 |
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