New IMAC Media Enabling Purification of Histidine-tagged Proteins Directly From Eukaryotic Cell Culture Supernatants

Immobilized metal affinity chromatography (IMAC) purification of secreted histidine-tagged proteins in eukaryotic cell culture supernatants is often problematic. Incompatibility with the cell culture media appears as stripping of the immobilized metal ions required for binding of target proteins. Th...

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Main Authors: Carlsson, Marianne, Granér, Therese, Algotsson, Mattias, Andersson, Lars C., Björner, Maria, Glad, Gunnar, Hörnsten, Lena, Le Greves, Pierre, Lindgren, Helena, Lindqvist, Staffan, Öberg, Katarina, Hedlund, Helena
Formato: Artigo
Idioma:Inglês
Publicado em: Association of Biomolecular Resource Facilities 2013
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Poster Session Abstracts
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3635365/
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