New IMAC Media Enabling Purification of Histidine-tagged Proteins Directly From Eukaryotic Cell Culture Supernatants

Immobilized metal affinity chromatography (IMAC) purification of secreted histidine-tagged proteins in eukaryotic cell culture supernatants is often problematic. Incompatibility with the cell culture media appears as stripping of the immobilized metal ions required for binding of target proteins. Th...

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Библиографические подробности
Главные авторы: Carlsson, Marianne, Granér, Therese, Algotsson, Mattias, Andersson, Lars C., Björner, Maria, Glad, Gunnar, Hörnsten, Lena, Le Greves, Pierre, Lindgren, Helena, Lindqvist, Staffan, Öberg, Katarina, Hedlund, Helena
Формат: Artigo
Язык:Inglês
Опубликовано: Association of Biomolecular Resource Facilities 2013
Предметы:
Poster Session Abstracts
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC3635365/
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