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The Influence of Histidine Tag Attachment on Picosecond Protein Dynamics
Poly-Histidine (His) affinity tags are routinely employed as a convenient means of purifying recombinantly expressed proteins. A tacit assumption is commonly made that His tags have little influence on protein structure and function. Attachment of a His tag to the N terminus of the robust globular p...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2011
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3133630/ https://ncbi.nlm.nih.gov/pubmed/21619030 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi2003923 |
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