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The Influence of Histidine Tag Attachment on Picosecond Protein Dynamics

Poly-Histidine (His) affinity tags are routinely employed as a convenient means of purifying recombinantly expressed proteins. A tacit assumption is commonly made that His tags have little influence on protein structure and function. Attachment of a His tag to the N terminus of the robust globular p...

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Bibliografski detalji
Glavni autori: Thielges, Megan C, Chung, Jean K., Axup, Jun Y., Fayer, Michael D.
Format: Artigo
Jezik:Inglês
Izdano: 2011
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3133630/
https://ncbi.nlm.nih.gov/pubmed/21619030
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi2003923
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