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Column Purification of Histidine-Tagged Proteins from Unclarified Samples
The histidine-tag is today the most commonly used tag for recombinant proteins. It is widely used to facilitate purification of recombinant expressed proteins, but can also be useful for detection purposes. For immobilized metal-ion affinity chromatography (IMAC) the most used metal ion is nickel (N...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Association of Biomolecular Resource Facilities
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3630661/ |
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