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Effective Identification of Akt Interacting Proteins by Two-Step Chemical Crosslinking, Co-Immunoprecipitation and Mass Spectrometry

Akt is a critical protein for cell survival and known to interact with various proteins. However, Akt binding partners that modulate or regulate Akt activation have not been fully elucidated. Identification of Akt-interacting proteins has been customarily achieved by co-immunoprecipitation combined...

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Detalhes bibliográficos
Main Authors: Huang, Bill X., Kim, Hee-Yong
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3629208/
https://ncbi.nlm.nih.gov/pubmed/23613850
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0061430
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