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Conformational changes in Akt1 activation probed by amide hydrogen/deuterium exchange and nano-electrospray ionization mass spectrometry

Amide hydrogen exchange coupled to nano-electrospray ionization mass spectrometry (nano-ESI-MS) has been used to identify and characterize localized conformational changes of Akt upon activation. Active or inactive Akt was incubated in D(2)O buffer, digested with pepsin, and analyzed by nano-ESI-MS...

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Détails bibliographiques
Auteurs principaux: Guo, Mingquan, Huang, Bill X., Kim, Hee-Yong
Format: Artigo
Langue:Inglês
Publié: 2009
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2752348/
https://ncbi.nlm.nih.gov/pubmed/19462409
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/rcm.4085
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