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Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

Intrinsically disordered proteins (IDPs) have long been a challenge to structural biologists due to their lack of stable secondary structure elements. Hydrogen-Deuterium Exchange (HDX) measured at rapid time scales is uniquely suited to detect structures and hydrogen bonding networks that are briefl...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Lento, Cristina, Zhu, Shaolong, Brown, Kerene A., Knox, Ruth, Liuni, Peter, Wilson, Derek J.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5564945/
https://ncbi.nlm.nih.gov/pubmed/28448048
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55464
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