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Chemical Mutagenesis of Vaccinia DNA Topoisomerase Lysine-167 Provides Insights to Catalysis of DNA Transesterification

Vaccinia DNA topoisomerase IB (TopIB) relaxes supercoils by forming and resealing a covalent DNA-(3′-phosphotyrosyl(274))-enzyme intermediate. Conserved active site side chains promote the attack of Tyr274 on the scissile phosphodiester via transition state stabilization and general acid catalysis....

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Bibliografiske detaljer
Main Authors: Yakovleva, Lyudmila, Shuman, Stewart
Format: Artigo
Sprog:Inglês
Udgivet: 2013
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3619185/
https://ncbi.nlm.nih.gov/pubmed/23317114
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi301643h
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