Isolation and Characterization of Antibody Fragments Selective for Specific Protein Morphologies from Nanogram Antigen Samples

We developed atomic force microscope (AFM) based protocols that enable isolation and characterization of antibody based reagents that selectively bind target protein variants using low nanogram amounts or less of unpurified starting material. We isolated single chain antibody fragments (scFvs) that...

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Detalhes bibliográficos
Main Authors: Kasturirangan, Srinath, Reasoner, Tim, Schulz, Philip, Boddapati, Shanta, Emadi, Sharareh, Valla, Jon, Sierks, Michael R.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3618510/
https://ncbi.nlm.nih.gov/pubmed/23359572
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/btpr.1698
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