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Use of microarray hybrid capture and next-generation sequencing to identify the anatomy of a transgene

Transgenic animals are extensively used to model human disease. Typically, the transgene copy number is estimated, but the exact integration site and configuration of the foreign DNA remains uncharacterized. When transgenes have been closely examined, some unexpected configurations have been found....

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Detalhes bibliográficos
Main Authors: DuBose, Amanda J., Lichtenstein, Stephen T., Narisu, Narisu, Bonnycastle, Lori L., Swift, Amy J., Chines, Peter S., Collins, Francis S.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3616733/
https://ncbi.nlm.nih.gov/pubmed/23314155
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gks1463
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