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Single-cell transcriptomics from human pancreatic islets: sample preparation matters

Single-cell RNA sequencing (scRNA-seq) of human primary tissues is a rapidly emerging tool for investigating human health and disease at the molecular level. However, optimal processing of solid tissues presents a number of technical and logistical challenges, especially for tissues that are only av...

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Dades bibliogràfiques
Publicat a:Biol Methods Protoc
Autors principals: Bonnycastle, Lori L, Gildea, Derek E, Yan, Tingfen, Narisu, Narisu, Swift, Amy J, Wolfsberg, Tyra G, Erdos, Michael R, Collins, Francis S
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2019
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6964677/
https://ncbi.nlm.nih.gov/pubmed/31984226
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/biomethods/bpz019
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