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Integrated view of genome structure and sequence of a single DNA molecule in a nanofluidic device

We show how a bird’s-eye view of genomic structure can be obtained at ∼1-kb resolution from long (∼2 Mb) DNA molecules extracted from whole chromosomes in a nanofluidic laboratory-on-a-chip. We use an improved single-molecule denaturation mapping approach to detect repetitive elements and known as w...

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Autors principals: Marie, Rodolphe, Pedersen, Jonas N., Bauer, David L. V., Rasmussen, Kristian H., Yusuf, Mohammed, Volpi, Emanuela, Flyvbjerg, Henrik, Kristensen, Anders, Mir, Kalim U.
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2013
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3612670/
https://ncbi.nlm.nih.gov/pubmed/23479649
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1214570110
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