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Single-molecule denaturation mapping of DNA in nanofluidic channels
Here we explore the potential power of denaturation mapping as a single-molecule technique. By partially denaturing YOYO®-1-labeled DNA in nanofluidic channels with a combination of formamide and local heating, we obtain a sequence-dependent “barcode” corresponding to a series of local dips and peak...
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| Autors principals: | , , , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
2010
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2922186/ https://ncbi.nlm.nih.gov/pubmed/20616076 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1007081107 |
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