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A general approach to break the concentration barrier in single-molecule imaging

Single-molecule fluorescence imaging is often incompatible with physiological protein concentrations, as fluorescence background overwhelms an individual molecule's signal. We solve this problem with a new imaging approach called PhADE (PhotoActivation, Diffusion, and Excitation). A protein of...

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Bibliografske podrobnosti
Main Authors: Loveland, Anna B., Habuchi, Satoshi, Walter, Johannes C., van Oijen, Antoine M.
Format: Artigo
Jezik:Inglês
Izdano: 2012
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3610324/
https://ncbi.nlm.nih.gov/pubmed/22961247
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2174
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