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A general approach to break the concentration barrier in single-molecule imaging
Single-molecule fluorescence imaging is often incompatible with physiological protein concentrations, as fluorescence background overwhelms an individual molecule's signal. We solve this problem with a new imaging approach called PhADE (PhotoActivation, Diffusion, and Excitation). A protein of...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2012
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3610324/ https://ncbi.nlm.nih.gov/pubmed/22961247 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2174 |
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