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A new method to customize protein expression vectors for fast, efficient and background free parallel cloning

BACKGROUND: Expression and purification of correctly folded proteins typically require screening of different parameters such as protein variants, solubility enhancing tags or expression hosts. Parallel vector series that cover all variations are available, but not without compromise. We have establ...

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Detalhes bibliográficos
Main Authors: Scholz, Judith, Besir, Hüseyin, Strasser, Claudia, Suppmann, Sabine
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3598636/
https://ncbi.nlm.nih.gov/pubmed/23410102
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-13-12
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