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FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method
BACKGROUND: Although a variety of methods and expensive kits are available, molecular cloning can be a time-consuming and frustrating process. RESULTS: Here we report a highly simplified, reliable, and efficient PCR-based cloning technique to insert any DNA fragment into a plasmid vector or into a g...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3207894/ https://ncbi.nlm.nih.gov/pubmed/21992524 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-11-92 |
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