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An Efficient Method to Prepare PCR Cloning Vectors

An improved procedure for preparing PCR cloning vectors was developed. This procedure includes the incorporation of adapters to create XcmI restriction enzyme sites in pBluescript II SK(+) vectors, digestion with XcmI followed by further digestion of the small fragment produced by XcmI digestion wit...

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Detalhes bibliográficos
Main Authors: Hong, Soon Gyu, Choi, Ji Young, Pryor, Barry M., Lee, Hong Kum
Formato: Artigo
Idioma:Inglês
Publicado em: The Korean Society of Mycology 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3749396/
https://ncbi.nlm.nih.gov/pubmed/23983541
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4489/MYCO.2009.37.3.240
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