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A Localized In-Situ Hydrogel-Mediated Protein Digestion and Extraction Technique For On-Tissue Analysis

A simultaneous on-tissue proteolytic digestion and extraction method is described for the in-situ analysis of proteins from spatially distinct areas of a tissue section. The digestion occurs on-tissue within a hydrogel network, and peptides extracted from this gel are identified with liquid chromato...

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Detalhes bibliográficos
Main Authors: Harris, Glenn A., Nicklay, Joshua J., Caprioli, Richard M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3595595/
https://ncbi.nlm.nih.gov/pubmed/23402265
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac3031493
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