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Histology-Directed Microwave Assisted Enzymatic Protein Digestion for MALDI MS Analysis of Mammalian Tissue
[Image: see text] This study presents on-tissue proteolytic digestion using a microwave irradiation and peptide extraction method for in situ analysis of proteins from spatially defined regions of a tissue section. The methodology utilizes hydrogel discs (1 mm diameter) embedded with trypsin solutio...
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| Veröffentlicht in: | Anal Chem |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
American
Chemical
Society
2014
|
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4287167/ https://ncbi.nlm.nih.gov/pubmed/25427280 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac503479a |
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