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A Localized In-Situ Hydrogel-Mediated Protein Digestion and Extraction Technique For On-Tissue Analysis
A simultaneous on-tissue proteolytic digestion and extraction method is described for the in-situ analysis of proteins from spatially distinct areas of a tissue section. The digestion occurs on-tissue within a hydrogel network, and peptides extracted from this gel are identified with liquid chromato...
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| Main Authors: | , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
2013
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3595595/ https://ncbi.nlm.nih.gov/pubmed/23402265 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac3031493 |
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