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Bacterial Expression and Purification of the Amyloidogenic Peptide PAPf39 for Multidimensional NMR Spectroscopy

PAPf39 is a 39 residue peptide fragment from human prostatic acidic phosphatase that forms amyloid fibrils in semen. These fibrils have been implicated in facilitating HIV transmission. To enable structural studies of PAPf39 by NMR spectroscopy, efficient methods allowing the production of milligram...

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Autores principales: Shanmuganathan, Aranganathan, Bishop, Anthony C., French, Kinsley C., McCallum, Scott A., Makhatadze, George I.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2013
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3594438/
https://ncbi.nlm.nih.gov/pubmed/23314347
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2013.01.003
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