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Preparation of isotopically labeled ribonucleotides for multidimensional NMR spectroscopy of RNA.
A general method for large scale preparation of uniformly isotopically labeled ribonucleotides and RNAs is described. Bacteria are grown on isotopic growth medium, and their nucleic acids are harvested and degraded to mononucleotides. These are enzymatically converted into ribonucleoside triphosphat...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1992
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| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC334179/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1383928/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.17.4515 |
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