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Deletion and replacement of the mouse adult beta-globin genes by a "plug and socket" repeated targeting strategy.

We describe a two-step strategy to alter any mouse locus repeatedly and efficiently by direct positive selection. Using conventional targeting for the first step, a functional neo gene and a nonfunctional HPRT minigene (the "socket") are introduced into the genome of HPRT- embryonic stem (ES) cells...

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Publicado no:Mol Cell Biol
Principais autores: Detloff, P J, Lewis, J, John, S W, Shehee, W R, Langenbach, R, Maeda, N, Smithies, O
Formato: Artigo
Idioma:Inglês
Publicado em: Taylor & Francis 1994
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC359224/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7935410/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.14.10.6936-6943.1994
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