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Deletion and replacement of the mouse adult beta-globin genes by a "plug and socket" repeated targeting strategy.

We describe a two-step strategy to alter any mouse locus repeatedly and efficiently by direct positive selection. Using conventional targeting for the first step, a functional neo gene and a nonfunctional HPRT minigene (the "socket") are introduced into the genome of HPRT- embryonic stem (...

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Main Authors: Detloff, P J, Lewis, J, John, S W, Shehee, W R, Langenbach, R, Maeda, N, Smithies, O
Format: Artigo
Jezik:Inglês
Izdano: 1994
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC359224/
https://ncbi.nlm.nih.gov/pubmed/7935410
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